KEAP1 Knockout cell line (THP-1)

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KEAP1 Knockout cell line (THP-1) Order

KEAP1 Knockout cell line (THP-1)
Catalog#
YKO-HT0348
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
KEAP1 Knockout cell line (THP-1)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
KEAP1 Knockout cell line (THP-1)
Species
Gene Name
KEAP1
Gene ID
9817
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the KEAP1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, KEAP1 gene knockout KEAP1 Knockout cell line (THP-1) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
KEAP1 Knockout cell line (THP-1)
KEAP1 Knockout cell line (THP-1)
Catalog#
YKO-HT0348
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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